Monoclonal Antibody Production

CUSTOM HYBRIDOMA SERVICE: $8965/project

Phase I: Immunogen design and Immunization (8-9 wks).

• Design and preparation of custom peptide immunogens or protein antigens.
• Site directed KLH/BSA conjugation for peptides.
• Multiple immunizations of Balb/c mice (n = 5).
• Test bleed antibody titering and qualification prior to the final boost and cell fusion in Phase II.

Phase II: Cell fusion and parental clone screening (3-wks).

• Approximately 6-8 weeks after initial immunization, mouse with the highest antisera titer will be given a final boost for cell fusion.
• Fused cell clones are screened by ELISA.
• Potential parental clones will be expanded into 24-well plates to harvest relatively large amount of tissue culture supernatant for further antibody screening (ELISA, WB or IHC).
• Shipment of up to 12 supernatants (0.5-1 ml/per parental clone).
• Customer must feedback within 1 week to ensure viability of clones.

Phase III: Monoclonal selection and cryogenic vial preservation (3-wks).

• Monoclonal selection of the best 1-3 clones will be performed (based on the customer’s choice).
• Subclone(s) with high specificity and antibody titer will be expanded and cryopreserved.
• 10 ml of supernatant and the cryopreserved vials (3 vials) from each clone will be shipped to customers.
• Customer must feedback within 1 week to ensure viability of clones.

Phase IV: Large scale antibody production (3-6wks) (optional)

• In vivo ascites production (20-40 ml of ascites each clone) or In vitro Bioreactor antibody production (up to 120 ml of supernatant each clone).
• Monoclonal antibody purification from ascites or culture supernatant using affinity chromatography. ELISA and SDS-PAGE will be performed to ensure the quality of the purified monoclonal antibody.
• AnaSpec provides both small and large scale antibody production with flexible cost adjustment.

IMPORTANT NOTE: AnaSpec will store the parental clones and subclones for 3 months only. Unless contacted by customer within the 3-month period, these clones will be discarded. Storage fee applies if customers choose to keep the clones at AnaSpec.

*On-line status reports are available for your custom peptide or antibody projects. These reports are password protected and provide the following benefits:

• Easy accessibility via the Internet at your convenience
• Reports of custom projects updated regularly
• Improved information exchange enabling better planning of research projects

Click the links below for detailed information:

For a demonstration of the feature:
1. Visit www.anaspec.com
2. Click on My Account
3. Enter “demo” for both Customer ID and Password