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Fluorescent Dyes  >  Enzyme Detection Reagents  >>  Collagen (Type I), FITC conjugated *Water Soluble*

Product Name Collagen (Type I), FITC conjugated *Water Soluble*
Size 1 mg
Catalog # AS-85111
US$ $247
Description

AnaSpec offers two types of fluoresceinated collagen (type I). This product is water soluble and is used for quick fluorometric measurement of collagenase activity. In this protease substrate, collagen is heavily labeled with FITC, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. This fluoresceinated collagen is useful for detecting MMP-1 activity.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Ref: Sela MN, et al. (2003). Enzymatic degradation of collagen-guided tissue regeneration membranes by periodontal bacteria. Clin Oral Implants Res 14, 263-8; Baici A, et al. (1980). A handy assay for collagenase using reconstituted fluorescein-labeled collagen fibrils. Anal Biochem 108, 230-2; Sugiyama K, et al. (1980). A simple and rapid assay for collagenase activity using fluorescence-labeled substrate.Kurume Med J 27, 63-9.
Molecular Weight N/D
Spectral Properties Abs/Em = 492/515 nm
Solvent System Water soluble
     
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