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Fluorescent Dyes  >  Enzyme Detection Reagents  >>  Casein, TAMRA Conjugated

Product Name Casein, TAMRA Conjugated
Size 5 mg
Catalog # AS-85101
US$ $181
Description

In the intact substrate, casein is heavily labeled with TAMRA, resulting in almost total quenching of the conjugate's fluorescence. Compared to the fluoresceinated casein, TAMRA-labeled casein demonstrates pH-insensitive fluorescence. It is readily used for continuous fluorometric measurement of protease activity. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly red fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. The lightly TAMRA-labeled casein may be useful for a continuous assay if monitored by fluorescence polarization.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Ref: Arthur JS and Mykles DL (2000). Calpain zymography with casein or fluorescein isothiocyanate casein. Methods Mol Biol. 144, 109-16; Farmer WH and Yuan ZY (1991). A continuous fluorescent assay for measuring protease activity using natural protein substrate. Anal Biochem 197, 347-52; Twining SS (1984). Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes. Anal Biochem 143, 30-4.
Molecular Weight ~26000
Spectral Properties Abs/Em = 540/570 nm
Solvent System water
     
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