Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AMC-based substrates yield blue fluorescence upon protease cleavage (Ex/Em=354 nm/442 nm). They are widely used to monitor caspase activity.
The SensoLyte® Caspase Substrate Kit contains a series of AMC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. It provides the best solution for identifying a suitable substrate for designing AMC-based caspase assays. All the components in the kit are available in bulk package.
The kit contains: • 8 AMC caspase substrates • Assay buffer • AMC (fluorescence reference standard for calibration) • A detailed protocol