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Fluorescent Dyes  >  Reactive Fluorescent Dyes  >>  ABD-F

Product Name ABD - F
[4 - Fluoro - 7 - aminosulfonylbenzofurazan]
Size 10 mg
Catalog # 81401
US$ $88
Description

ABD-F has a benzofurazan moiety that readily reacts with thiol compounds to generate highly fluorescent products. The ABD-F/thiol adduct has absorption maximum at 389 nm and fluorescence maximum at 513 nm. The reaction rate of ABD-F is 30 times faster than that of SBD-F. It is widely used for HPLC derivatizations of thiol compounds.

Storage -20C desiccated and protected from light
References Uchiyama S, et al. (2001). Fluorogenic and fluorescent labeling reagents with a benzofurazan skeleton. Biomed Chromatog 15, 295-31); Treuheit MJ and Kirley TL (1993). Reversibility of cysteine labeling by 4-(aminosulfonyl)-7-fluoro-2,1,3- benzoxadiazole. Anal Biochem 212, 138-42.
Molecular Weight 217.18
Spectral Properties Abs/Em = 315/none nm
Solvent System DMF or DMSO
Product Citations Cuneo, M. et al. (2010). Oxidation state of the XRCC1 N-terminal domain regulates DNA polymerase β binding affinity. PNAS 107, 6805.
Vercillo, NC. et al. Protein Sci. 16, 362 (2007).
     
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