ABD - F [4 - Fluoro - 7 - aminosulfonylbenzofurazan]
ABD-F has a benzofurazan moiety that readily reacts with thiol compounds to generate highly fluorescent products. The ABD-F/thiol adduct has absorption maximum at 389 nm and fluorescence maximum at 513 nm. The reaction rate of ABD-F is 30 times faster than that of SBD-F. It is widely used for HPLC derivatizations of thiol compounds.
Uchiyama S, et al. (2001). Fluorogenic and fluorescent labeling reagents with a benzofurazan skeleton. Biomed Chromatog 15, 295-31); Treuheit MJ and Kirley TL (1993). Reversibility of cysteine labeling by 4-(aminosulfonyl)-7-fluoro-2,1,3- benzoxadiazole. Anal Biochem 212, 138-42.