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Fluorescent Dyes  >  Reactive Fluorescent Dyes  >>  Fluorescein-5-maleimide

Product Name Fluorescein - 5 - maleimide
CAS 75350-46-8
Size 25 mg
Catalog # 81405
US$ $159
Purity ≥95% by HPLC

Maleimides are among the most frequently used reagents for thiol modification. In most proteins, the site of reaction is at cysteine residues that either are intrinsically present or result from reduction of cystines. Unlike iodoacetamides, maleimides do not react with histidines and methionines under physiological conditions. Fluorescein-5-maleimide is one of the most popular fluorescent dyes for thiol modifications of proteins.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Winograd E, et al. (2004). Chemical modifications of band 3 protein affect the adhesion of Plasmodium falciparum-infected erythrocytes to CD36. Mol Biochem Parasitol 136, 243-8; van der Sluis EO, et al. (2002). SecY-SecY and SecY-SecG contacts revealed by site-specific crosslinking. FEBS Lett 527, 159-65.
Molecular Weight 427.36
Spectral Properties Abs/Em = 493/515 nm
Solvent System DMF or DMSO
Product Citations Moreno, ML. et al. (2014). Disulfide stress: a novel type of oxidative stress in acute pancreatitis. Free Radical Biol Med 70, 265.
Kamaduri, H, er al. (2013). Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3. elife2, e00828. doi: 10.7554/eLife.00828.
Newbern, J. et al. (2007). c-Jun N-terminal kinase signaling regulates events associated with both health and degeneration in motoneurons. Neurosci 147, 680.
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