Maleimides are among the most frequently used reagents for thiol modification. In most proteins, the site of reaction is at cysteine residues that either are intrinsically present or result from reduction of cystines. Unlike iodoacetamides, maleimides do not react with histidines and methionines under physiological conditions. Fluorescein-5-maleimide is one of the most popular fluorescent dyes for thiol modifications of proteins.
Winograd E, et al. (2004). Chemical modifications of band 3 protein affect the adhesion of Plasmodium falciparum-infected erythrocytes to CD36. Mol Biochem Parasitol 136, 243-8; van der Sluis EO, et al. (2002). SecY-SecY and SecY-SecG contacts revealed by site-specific crosslinking. FEBS Lett 527, 159-65.
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Material Safety Data Sheets (MSDS)
