HiLyte Fluor™ 750 amine is a carbonyl-reactive fluorescent labeling dye. Spectrally similar to Cy7 dye, HiLyte Fluor™ 750 amine is the longest-wavelength carbonyl-reactive HiLyte Fluor™ dye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte Fluor™ 647, HiLyte Fluor™ 680 or allophycocyanin (APC), facilitating multicolor analysis. With a peak excitation at ~754 nm, conjugates of HiLyte Fluor™ 750 dyes are well excited by a xenon-arc lamp or dye-pumped lasers operating in the 720–750 nm range.
Extinction Coefficient (M-1cm-1): 275,000 Fluorescence quantum yield: 0.12 Fluorescence Life Time (ns): 0.7