In general, pyrene fluorophores exhibit structured spectra. The absorption maximum is usually centered around 340 nm, and the emission maximum is usually about 380 nm with excimer emission at about 470 nm. 1-Pyrenebutanoic acid is an UVexcitable fluorophore that has concentration-dependent fluorescence due to the formation of excimer. It is widely used to develop ratiometric fluorescent membrane probes.
Paproski RE, et al. (2002). Selective fluorometric detection of polyamines using micellar electrokinetic chromatography with laser-induced fluorescence detection. J Chromatogr A 946, 265-73; Martins J and Melo E (2001). Molecular mechanism of lateral diffusion of py(10)-PC and free pyrene in fluid DMPC bilayers. Biophys J 80, 832-40.
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