ROX dyes have longer excitation and emission wavelengths than the other ‘conventional’ rhodamines. These dyes are used to label peptides, proteins, and other biological ligands. 5-ROX, 6-ROX and their mixture 5(6)-ROX are used to label biomolecules by EDC-mediated reactions.
Hahn M, et al. (2001). Influence of fluorophore dye labels on the migration behavior of polymerase chain reaction-amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis 22, 2691-700; Li Y and Glazer AN (1999). Design, synthesis and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes. Bioconjug Chem 10, 241-5.