5 - ROX, SE 5 - Carboxy - X - rhodamine, succinimidyl ester; 5 - ROX, NHS ester
Single isomers of ROX are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purifications that are often required in bioconjugations. Variable ratios of the 5- and 6-isomers often cause complications in the interpretation of labeling results and assay performances.
Dominko T, et al. (2000). Dynamic imaging of the metaphase II spindle and maternal chromosomesin bovine oocytes: implications for enucleation efficiency verification, avoidance of parthenogenesis, and successful embryogenesis. Biol Reprod 62, 150-4; Gorbsky GJ, et al. (1990). Microtubules in the metaphase-arrested mouse oocyte turn over rapidly. Proc Natl Acad Sci USA 87, 6049-53.