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Assay Kits  >  MMP Assay Kits  >>  SensoLyte® 520 MMP-1 Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 MMP - 1 Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 71150
US$ $435
Description

MMP-1 (interstitial collagenase, fibroblast collagenase) is an extracellular protease. This enzyme plays a significant role in cancer, arthritis and other diseases, and has been identified as a drug-screening target. The SensoLyte® 520 MMP-1 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrate for continuous measurement of enzyme activity. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-1, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format. Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-1 activity, please choose the SensoLyte® Plus MMP-1 Assay Kit, Cat# 72012.

Kit size:100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Brochure
Storage -20°C
Product Citations Herrera, I. et al. (2013). Matrix Metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption. J Biol Chem 288, 25964. doi: 10.1074/jbc.M113.459784.
Yang, SW. et al. (2011). Matrix metalloproteinase-1 induces cleavage of exogenous alphaB-crystallin transduced by a cell-penetrating peptide. J Cell Biochem 10.1002/jcb.23167.
Dayoub, R. et al. (2011). Liver regeneration associated protein (ALR) exhibits antimetastatic potential in hepatocellular carcinoma. Mol Med 17, 221.
Spiga, MG. et al. (2010). Development of a gene therapy virus with a glucocorticoid-inducible MMP1 for the treatment of steroid glaucoma. Invest Ophthalmol Vis Sci 51, 3029.
Yi, F. et al. (2006). Inhibition of ceramide–redox signaling pathway blocks glomerular injury in hyperhomocysteinemic rats. Kidney Intl 70, 88.
     
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