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Assay Kits  >  MMP Assay Kits  >>  SensoLyte® 520 MMP-12 Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 MMP - 12 Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-71157
US$ $435
Description

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-12 (macrophage elastase) is expressed in activated macrophages and may play a role in smoke-induced emphysema. SensoLyte® 520 MMP-12 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-12 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13 and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-12, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Kit size:100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Brochure
Storage -20°C
Product Citations Yokose, U. et al. (2012). The endogenous protease inhibitor TIMP-1 mediates protection and recovery from cutaneous photodamage. J Invest Derma doi:10.1038/jid.2012.204
Haq, I. et al. (2011). Matrix metalloproteinase-12 (MMP-12) SNP affects MMP activity, lung macrophage infiltration and protects against emphysema in COPD. Thorax 10.1136/thx.2011.159087.
Margheri, F. et al. (2010). Modulation of the angiogenic phenotype of normal Systemic Sclerosis endothelial cells by gain-loss of function of PTX3 MMP12. Arthritis Rheumatism 10.1002/art.27522.
     
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