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Fluorescent Dyes  >  Fluorescent Physiological and Neurological Indicators  >>  Calcein, AM *UltraPure Grade*, 5 mM solution in anhydrous DMSO

Product Name Calcein, AM *UltraPure Grade*, 5 mM solution in anhydrous DMSO
CAS 148504-34-1
Size 200 µL
Catalog # 89203
US$ $169
Purity ≥95% by HPLC
Description

Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein, AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells. Hela cells stained with AnaSpec’s Calcein AM fluorescent dye (Cat# 89201, 89202, 89203 and 89204).

MCF-7 cells grown in 8-well slide chamber for 2 days. Cells were washed twice before adding 1 uM Calcein AM (AnaSpec cat# 89202). Images acquired every 3 seconds and total elapsed time on video is 12 min.

Detailed Information Material Safety Data Sheets (MSDS)
Storage -20°C desiccated and protected from light
Molecular Weight 994.9
Spectral Properties Abs/Em = 494/517 nm
Solvent System DMSO
Product Citations Robinson, M. et al. (2011). Screening of hepatitis C virus inhibitors using genotype 1a HCV replicon cell lines. Curr Prot Microbiol doi: 10.1002/9780471729259.mc1707s22.
Delaunay, JL. et al. (2009). A missense mutation in ABCB4 gene involved in progressive familial intrahepatic cholestasis type 3 leads to a folding defect that can be rescued by low temperature. Hepatology 49, 1218.
Zhang, K-H. et al. (2009). Ferritin heavy chain–mediated iron homeostasis and subsequent increased reactive oxygen species production are essential for epithelial-mesenchymal transition. Cancer Res. 10.1158/0008-5472.CAN-09-0112.
     
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