Assay Kits  >  Viral Protease Assay Kits - HIV, HCV, WNV  >>  SensoLyte^® 620 HIV Protease Assay Kit *Fluorimetric*

SensoLyte® 620 HIV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor™ TR (fluorophore) and QXL™ 610 (quencher) for continuous measurement of the enzyme activities. In the intact FRET peptide, the fluorescence of Hylite Fluor™ TR is quenched by QXL™ 610. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of Hylite Fluor™ TR is recovered and can be continuously monitored at excitation/emission = 590 nm/620 nm. With superior fluorescence quantum yield and longer emission wavelength, HiLyte Fluor™ TR/QXL™ 610-based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: • HiLyte Fluor™TR/QXL™610 FRET peptide substrate (Ex/Em=590/620 nm upon cleavage) • Assay buffer • HIV protease inhibitor • Fluorescence reference standard for calibration • A detailed protocol

Kit size: 100 assays