This peptide* induced anti-MOG(35-55) autoantibody production in C57BL/6 mice with 100% incidence (10/10); providing quantitative support for the suitability of the MOG peptide for in vivo anti-MOG(35-55) autoantibody study. Cysteine containing MOG (35-55) peptide induced polyclonal anti-MOG(35-55) antibody production in rabbits with high specificity, high affinity, and high titer indicating its high purity, as well as suitability for peptide-coated ELISA and Western blot assay. Using this mouse/rat MOG (35-55) peptide as a coating peptide, AnaSpec produced the industry’s first anti-MOG (35-55) ELISA kit for EAE study. The detection limit was measured to be as low as 50 pg of anti-MOG (35-55) autoantibody in serum or cerebrospinal fluid. After testing different lots of AnaSpec MOG peptides for ELISA coating, AnaSpec reported that the quality variation from lot to lot was minimal. *Tested for lot# 61918 & 71369