Cys(Npys) - TAT (47 - 57) C(Npys)YGRKKRRQRRR - NH2
This peptide corresponds to the protein transduction domain of the TAT protein and is synthesized with an activated cysteine residue C(Npys), wherein Npys is 3-Nitro-2-pyridinesulfenyl group and is used for activating S of cysteine and for rapid reaction when a thiol group is introduced. This kind of modification has been used to render this peptide as a cell penetrating and carrier peptide applicable in conjugation studies.
1, Bright R. et al. Neurosci lett. 441, 120-124 (2008) 2, Moultin HM. Et al. Bioconj Chem. 15(2):290-299 (2004).