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Assay Kits  >  Caspase Assay Kits  >>  SensoLyte® Homogeneous AnaRed Caspase-3/7 Assay Kit

Product Name SensoLyte® Homogeneous AnaRed Caspase - 3/7 Assay Kit
500 assays (96 - well plate)
Size 1 kit
Catalog # 71122
US$ $374

Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).

The SensoLyte® Homogeneous AnaRed Capase-3/7 Assay Kit uses Ac-DEVD-AnaRed as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AnaRed generates the AnaRed fluorophore has bright red fluorescence and can be detected at Ex/Em=537 nm/611 nm. The long excitation and emission wavelength of AnaRed™ is useful when apoptotic inducing compounds themselves have strong fluorescence at lower wavelength. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
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