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Peptides  >  Caspase Related Peptides  >>  Caspase 8 Substrate 1m, fluorogenic

Product Name Caspase 8 Substrate 1m, fluorogenic
Ac - IETD - AMC
Size 5 mg
Catalog # 25257-5
US$ $154
Description

AMC (7-amino-4-methylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AMC peptides by caspases generates strongly fluorescent AMC that is monitored fluorimetrically at 440-460 nm with excitation of 340-350 nm.

Caspase-8 substrate

Detailed Information Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Koeplinger KA, et al. (2000). Caspase 8: an efficient method for large-scale autoactivation of recombinant procaspase 8 by matrix adsorption and characterization of the active enzyme. Protein Expr Purif 18, 378-87; Garcia-Calvo M, et al. (1998). Inhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem 273, 32608-13; Thornberry NA, et al. (1997). A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. J Biol Chem 272, 17907-11; Rano TA, et al. (1997). A combinatorial approach for determining protease specificities: application to interleukin-1 beta converting enzyme (ICE). Chem Biol 4, 149-55; Margolin N, et al. (1997). Substrate and inhibitor specificity of interleukin-1 ?-converting enzyme and related caspases. J Biol Chem 272, 7223-8; Mizushima N, et al. (1996). Ceramide induces apoptosis via CPP32 activation. FEBS Lett 395, 267-71; Faubion WA, et al. (1999). Toxic bile salts induce rodent hepatocyte apoptosis via direct activation of Fas. J Clin Invest 103, 137-45.
Molecular Weight 675.6
Product Citations Lin-Shiau, S. et al. (2003). Induction of Apoptosis by Rosemary Polyphenols in HL-60 Cells. Orient Foods Herbs. Chapter DOI: 10.1021/bk-2003-0859.ch008.
Gukovskaya, AS. et al. J Biol Chem 277, 22595 (2002).
Yusuf, K. et al. Ped Research 52, 411 (2002).
     
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