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Fluorescent Dyes  >  Labeling and Detection of Peptides and Proteins  >>  5-FTSC

Product Name 5 - FTSC
[Fluorescein - 5 - thiosemicarbazide]
Size 25 mg
Catalog # 81505
US$ $66
Description

5-FTSC reacts with ketones to yield relatively stable hydrazones and with aldehydes to yield hydrazones that are somewhat less stable, though they may be formed faster (compared to ketones). These hydrazones are generally reduced with sodium borohydride (NaBH4) to further increase the stability of the linkage. 5-FTSC has been used to make a wide variety of biomolecules such as L-aspartase-a-decarboxylase, enzyme-oxidized live plant protoplasts, immunoglobulins, thrombin and antithrombin.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Ahn B, et al. (1987). Use of fluorescein hydrazide and fluorescein thiosemicarbazide reagents for the fluorometric determination of protein carbonyl groups and for the detection of oxidized protein on polyacrylamide gels. Anal Biochem 161, 245-57; Atha DH, et al. (1984). Interaction and thermal stability of fluorescent labeled derivatives of thrombin and antithrombin III. Biochim Biophys Acta 785, 1-6.
Molecular Weight 421.4
Spectral Properties Abs/Em = 492/516 nm
Solvent System DMF or DMSO
     
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