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Fluorescent Dyes  >  Fluorescence Technologies and Their Applications  >>  Thioflavin T *UltraPure Grade*

Product Name Thioflavin T *UltraPure Grade*
Size 1 g
Catalog # AS-88306
US$ $66
Description

The benzothiazole dye thioflavin T (ThT) is a classic amyloid stain for senile plaques containing bA4 peptide in Alzheimer's disease brain. ThT also binds rapidly and specifically to the anti-parallel b-sheet fibrils formed from synthetic b-amyloid (1-40), but does not bind to monomer or oligomeric intermediates. The fibrillar b-sheet-bound dye species undergoes a characteristic 120 nm red shift of its excitation spectrum that may be selectively excited at 450 nm, resulting in a fluorescence signal at 482 nm. ThT is a useful probe for the aggregated fibrillar state of b-amyloid (1-40) fibrils as the amyloid-specific fluorescence reports only fibrillar species. The binding of ThT does not interfere with the aggregation of this peptide into amyloid fibrils. The putative conformational changes detected by the ThT fluorescence suggest that small pharmacologic ligands can perturb and possibly dissociate Ab amyloid fibrils.

Detailed Information Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Ban T, et al. (2003). Direct observation of amyloid fibril growth monitored by thioflavin T fluorescence. J Biol Chem 278, 16462-5. Kung MP, et al. (2002). IMPY: an improved thioflavin-T derivative for in vivo labeling of -amyloid plaques. Brain Res 956, 202-10. De Ferrari GV, et al. (2001). Thioflavin T is a fluorescent probe of the acetylcholinesterase peripheral site that reveals conformational interactions between the peripheral and acylation sites. J Biol Chem 276, 23282-7.
Molecular Weight 318.9
Spectral Properties Abs/Em = 412/482 nm
Solvent System DMSO
Product Citations Pronchik, J. et al. (2010). In vitro formation of amyloid from α-synuclein is dominated by reactions at hydrophobic interfaces. J Am Chem Soc 132, 9797.
Stsiapura, V. et al. (2010). Charge transfer process determines ultrafast excited state deactivation of thioflavin T in low-viscosity solvents. J Phys Chem A 114, 8345.
Sulatskaya, A.I. et al. (2010). Spectral properties and factors determining high quantum yield of thioflavin T incorporated in amyloid fibrils. Spectroscopy 24, 169.
     
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