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Peptides  >  FRET Peptides  >>  360 MMP FRET Substrate I

Product Name 360 MMP FRET Substrate I
Dnp - PLGLWAr - NH2
Size 1 mg
Catalog # 27074
US$ $99
Purity % Peak Area By HPLC ≥ 95%
Description

A generic MMP fluorescent substrate, originally prepared as a substrate for fibroblast collagenase. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P1 bond, giving rise to a continuously recording fluorescence assay. The hydrolysis rates of the FRET peptide rival or exceed that of its natural protein substrates. This substrate also demonstrates that its water solubility in the typical MMP assay buffer exceeds the Km value for the enzyme, allowing accurate determination of the kinetic parameters. Abs/Em = 280/360 nm.

Detailed Information Datasheet
Storage -20C
References Ref: Santala, A. et al. FEBS Lett. 461, 153 (1999); Bickett, DM. et al. Anal. Biochem. 212, 58 (1993); Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991); Stack, M. et al. J. Biol. Chem. 264, 4277 (1989).
Molecular Weight 977.1
Sequence
(One-Letter Code)
Dnp-PLGLWAr-NH2
Sequence
(Three-Letter Code)
Dnp - Pro - Leu - Gly - Leu - Trp - Ala - D - Arg - NH2
Product Citations Spinale, F. et al. (2008). Dynamic changes in matrix metalloprotienase activity within the human myocardial interstitium during myocardial arrest and reperfusion. Circulation 118, S16.
     
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