NFF-2 is hydrolyzed 60 times more rapidly by MMP-3 (kcat/Km = 59,400 s-1 M-1) than MMP-1. However, it has little discrimination between MMP-3, MMP-2 (kcat/Km = 54,000 s-1 M-1), and MMP-9 (kcat/Km = 55,300 s-1 M-1). The substrate can also be cleaved by MMP-8 and 12. Abs/Em = 325/393 nm.
Ref: Bremer, C. et al. Acad. Radiol. 9 Suppl 2, S314 (2002); Nagase, H. et al. J. Biol. Chem. 269, 20952 (1994).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
Mca - Arg - Pro - Lys - Pro - Tyr - Ala - Nva - Trp - Met - Lys(Dnp) - NH2