Fluorescent Dyes  >  Labeling and Detection of Peptides and Proteins  >>  AnaTag™ HiLyte Fluor™ 488 Microscale Protein Labeling Kit

HiLyte Fluor™ 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte Fluor™488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein. HiLyte Fluor™ 488 is more photostable than FITC, providing researchers with additional time for capturing image. Its fluorescence can be detected at the emission wavelength of 523±10 nm when excited at 499±10 nm. The HiLyte Fluor™ 488-protein conjugates are very stable and suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte Fluor™ 488-protein conjugate. The entire process takes only half an hour. The kit is able to perform three conjugation reactions with each reaction labeling up to 200 µg IgG.

Kit Size: 3 labeling reactions (3 x 200 µg protein)