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Assay Kits  >  MMP Assay Kits  >>  SensoLyte® 520 MMP - 14 Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 MMP - 14 Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 72025
US$ $435
Description

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-14 (MT1-MMP) is the membrane bound MMP. When expressed in cells, the catalytic and the hemopexin domains of MMP-14 become exposed on cell surfaces. This enzyme plays an important role in tumor invasion. SensoLyte® 520 MMP-14 Assay Kit uses a 5-FAM (fluorophore) and QXL®520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon the cleavage of the FRET peptide by MMP-14, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL®520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Kit size: 100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Brochure
Storage -20°C
Product Citations Kang, H. et al. (2012). Fluid shear stress and sphingosine 1-phosphate activate calpain to promote membrane type 1 matrix metalloproteinase (MT1-MMP) membrane translocation and endothelial invasion into three-dimensional collagen. J Biol Chem 286, 42017. doi: 10.1074/jbc.M111.290841.
Mahimkar, R. et al. (2011). Graded activation of the MEK1/MT1-MMP axis determines renal epithelial cell tumor phenotype. Carcinogenesis 32, 1806
Sugimoto, K. et al. (2009). LOX-1-MT1-MMP axis is crucial for RhoA and Rac1 activation induced by oxidized low-density lipoprotein in endothelial cells. Cardiovasc Res doi:10.1093/cvr/cvp177.
     
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