▀-Secretase (BACE1) is a key enzyme involved in the production of A▀ peptides found in extracellular amyloid plaques of Alzheimerĺs disease (AD). The enzyme has been implicated as an excellent target for anti-amyloid therapy of AD. This FRET peptide is an internally quenched fluorescent substrate based on the ▀-Secretase-cleavable peptide sequence. Upon cleavage by ▀-Secretase, the Mca fragment is liberated from the proximity quenching effect of Dnp (2,4-dinitrophenyl), Abs/Em=325/393 nm.
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
Mca - Glu - Val - Lys - Val - Asp - Ala - Glu - Phe - Lys(Dnp) - OH