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Fluorescent Dyes  >  Enzyme Detection Reagents  >>  HCV Protease FRET Substrate (RET S1)

Product Name HCV Protease FRET Substrate (RET S1)
Ac - DE - D(Edans) - EE - Abu - ψ - [COO] - AS - K(Dabcyl) - NH2
Size 0.25 mg
Catalog # 22991-025
US$ $181
Purity % Peak Area By HPLC ≥ 95%
Description

This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm.

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Detailed Information Datasheet
Storage -20C
References Lin, C. et al. J. Biol. Chem. 10, 1074 (2004); Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999); Liu, Y. et al. Anal. Biochem. 267, 331 (1999); Taliani, M. et al. Anal. Biochem. 240, 60 (1996).
Molecular Weight 1548.6
Sequence
(One-Letter Code)
Ac-DE-D(Edans)-EE-Abu-ψ-[COO]-AS-K(Dabcyl)-NH2
Sequence
(Three-Letter Code)
Ac - Asp - Glu - Asp(EDANS) - Glu - Glu - Abu - ψ - [COO] - Ala - Ser - Lys(DABCYL) - NH2
Product Citations Beran, R. et al. J Virol 83, 3268 (2009).
Lin, T. et al. Antimicrob Agents Chemother 53, 1377 (2009).
Pan, K. et al. Antimicrob Agents Chemother. 10 (2009).
Phan, T. et al. J Virol 83, 8379 (2009).
Beran, R. et al. J Biol Chem 283, 29929 (2008).
Zhou, Y. et al. Antimicrob Agents Chemother 52, 110 (2008).
Zhou, Y. et al. (2007). Phenotypic and Structural Analyses of Hepatitis C Virus NS3 Protease Arg155 Variants SENSITIVITY TO TELAPREVIR (VX-950) AND INTERFERON α. J Biol Chem 282, 22619.
Zhou, Y. et al. (2007). PHENOTYPIC CHARACTERIZATION OF VAL36 RESISTANT VARIANTS OF HEPATITIS C VIRUS NS34A SERINE PROTEASE. Antimicrob Agents Chemother doi:10.1128/AAC.00863-07.
Perni, R. et al. Antimicrob Agents Chemother 50, 899 (2006).
Ferreon, J. et al. J Biol Chem 280, 20483 (2005).
Lin, C. et al. J Biol Chem 279, 17508 (2004).
     
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