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Amino Acids  >  Synthesis Reagents, Resins & Accessories  >  Synthesis Reagents  >  Labeling Reagents  >>  5(6)-FAM, SE

Product Name 5(6) - FAM, SE
5 - (and - 6) - Carboxyfluorescein, succinimidyl ester; 5(6) - FAM, NHS ester
Size 25 mg
Catalog # AS-81006
US$ $66
Description

Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Hahn M, et al. (2001). Influence of fluorophore dye labels on the migration behavior of polymerase chain reaction-amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis 22, 2691-700; Sanders SJ (2000). Factor V Leiden genotyping using real-time fluorescent polymerase chain reaction. Mol Cell Probes 14, 249-53; Brandis JW (1999). Dye structure affects Taq DNA polymerase terminator selectivity. Nucleic Acids Res 27, 1912-8.
Molecular Weight 473.39
Molecular Formula C25H15NO9
Spectral Properties Abs/Em = 494/519 nm
Solvent System DMF or DMSO
Product Citations Williamson, Patrick. et al. PloS One. 2, 729 (2007).
     
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