Kinase Substrates Library, Group I, biotinylated, 180 distinct peptide mixtures
This positional scanning synthetic peptide combinatorial library (PS-SPCL) has been used in rapid identification of serine/threonine kinase phosphorylation motif. The use of this Group I of 180 distinct peptide mixtures, in combination with Group II of 18 distinct peptide mixtures (cat# 62335), can provide sequence preference information of the different kinases. Amount provided is 1 mg of peptide mixture x 180 vials.
X = degenerate mixture of the 17 natural amino acids excluding cysteine, serine, and threonine. LC = "long chain" version with an additional aminohexanoic acid spacer between the biotin and lysine side chain. S/T = equimolar mixture of serine and threonine. Z = fixed position varied between the 20 natural amino acids.
Turk, BB. et al. Nature Protocols1, 375 (2006); Hutti, JE. et al. Nature Methods1, 27 (2004); Pinilla, C. et al. Biotechniques13, 901 (1992).