Peptides  >  Kinases/Phosphatase Substrates  >  Kinase Substrate Library  >>  Kinase Substrates Library, Group I, biotinylated, 180 distinct peptide mixtures

This positional scanning synthetic peptide combinatorial library (PS-SPCL) has been used in rapid identification of serine/threonine kinase phosphorylation motif. The use of this Group I of 180 distinct peptide mixtures, in combination with Group II of 18 distinct peptide mixtures (cat# 62335), can provide sequence preference information of the different kinases. Amount provided is 1 mg of peptide mixture x 180 vials.

Sequences:
Y-A-Z-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH₂
Y-A-X-Z-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH₂
Y-A-X-X-Z-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH₂
Y-A-X-X-X-Z-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH₂
Y-A-X-X-X-X-Z-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH₂
Y-A-X-X-X-X-X-S/T-Z-X-X-X-A-G-K-K(LC-Biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-Z-X-X-A-G-K-K(LC-Biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-X-Z-X-A-G-K-K(LC-Biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-X-X-Z-A-G-K-K(LC-Biotin)-NH₂

X = degenerate mixture of the 17 natural amino acids excluding cysteine, serine, and threonine.
LC = "long chain" version with an additional aminohexanoic acid spacer between the biotin and lysine side chain.
S/T = equimolar mixture of serine and threonine.
Z = fixed position varied between the 20 natural amino acids.