Peptides  >  Kinases/Phosphatase Substrates  >  Kinase Substrate Library  >>  Kinase Substrates Library, Group II, biotinylated, 18 distinct peptide mixtures

This positional scanning synthetic peptide combinatorial library (PS-SPCL) has been used in rapid identification of serine/threonine kinase phosphorylation motif. The use of this Group II of distinct 18 peptide mixtures, in combination with Group I of 180 distinct peptide mixtures (cat# 62017-1 or cat# 62017-5), can provide sequence preference information of different kinases. Amount provided is 1 mg of peptide mixture x 18 vials.


Sequences:
Y-A-pT-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-pT-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-pT-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-pT-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-pT-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-pT-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-pT-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-X-pT-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-X-X-pT-A-G-K-K(LC-biotin)-NH₂

Y-A-pY-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-pY-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-pY-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-pY-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-pY-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-pY-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-pY-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-X-pY-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-X-X-pY-A-G-K-K(LC-biotin)-NH₂

X = degenerate mixture of the 17 natural amino acids excluding cysteine, serine, and threonine.
LC = "long chain" version with an additional aminohexanoic acid spacer between the biotin and lysine side chain.
S/T = equimolar mixture of serine and threonine. pT is phosphothreonine, and pY is phosphotyrosine.