Kinetic studies showed that the Km of YIYGSFK for p60c-src was 55 µM. It is about 6.4-fold lower than a peptide derived from p34cdc2 [cdc2(6-20), KVEKIGEGTYGVVYK] and has been reported to be a specific and efficient substrate for the Src-family protein tyrosine kinases. Comparison of the specificity of YIYGSFK and cdc2 (6-20) as a substrate for various Src-family and non-Src-family protein tyrosine kinases suggests that YIYGSFK is a much more specific and efficient substrate for the Src-family protein tyrosine kinases.
Ref: Lam, KS. et al. Int. J. Pept. Protein Res. 45, 587 (1995); Hunter, T. Harvey Lect. 94, 81 (1998).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
5 - TMR - Tyr - Ile - Tyr - Gly - Ser - Phe - Lys - OH