This sequence is N-ethyl-maleimide-sensitive factor (NSF) peptide connected to 11 amino acid cell permeable human immunodeficiency virus (HIV) transactivating regulatory protein (TAT) domain by Gly-Gly-Gly spacer. This peptide contains NSF domain extending from amino acids 222 to 243, which is directly amino-terminal of the Walker A motif of the D1 domain of NSF. ATPase assay shows that TAT-NSF222 inhibits NSF ATPase activity.
Matsushita, K. et al. Mol. Pharmacol. 67,1137 (2005).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Tyr - Gly - Arg - Lys - Lys - Arg - Arg - Gln - Arg - Arg - Arg - Gly - Gly - Gly - Leu - Asp - Lys - Glu - Phe - Asn - Ser - Ile - Phe - Arg - Arg - Ala - Phe - Ala - Ser - Arg - Val - Phe - Pro - Pro - Glu - OH