This amino acids 667 to 676 fragment of the amyloid precursor protein (APP) is a beta secretase substrate containing an Mca/ Dap(Dnp) FRET pair. Beside being cleaved by beta-site APP cleavage enzyme (BACE) between the Met and Asp residues, it can also be cleaved by the enzyme thimet oligopeptidase (TOP), Ex/Em=330 nm/390 nm.
Koike, H. et al. J. Biochem. 126, 235 (1999), Grüninger-Leitch, F. et al. J. Biol. Chem. 277, 4687 (2002), Verheijen, J. et al. Clin. Chem. 52, 1168 (2006).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
Mca - Ser - Glu - Val - Lys - Met - Asp - Ala - Glu - Phe - Arg - DAP(DNP) - NH2