This GluR2<Sub>3Y peptide was used in ELISA cell-surface assay for the insulin-stimulated endocytosis of native AMPA receptors in cultured hippocampal neurons. GluR23Y prevented any insulin-induced reduction. The blockade of insulin action was observed when the GluR2<Sub>3Y peptide was delivered into neurons by fusing it to the membrane transduction domain of HIV-1.
Ahmadian, G. et al. EMBO J. 23, 1040 (2004), Wang, D. et al. J. Biol. Chem. 279, 41267 (2004).