Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases involved in both normal and diseased tissue remodeling. MMP-13 (collagenase-3) can degrade quite a few extracellular matrix proteins, including collagens, gelatin, aggrecan, perlecan and fibronectin. The SensoLyte® MMP-13 ELISA Kit is an immunoassay for the quantification of total human MMP-13 (pro and active forms) in serum, plasma, cell culture supernates and urine. The assays are performed in a convenient 96-well microplate format. Kit contains 96-well coated plate, recombinant human MMP-13 standard, detection antibody, diluents, color reagent, HRP conjugated streptavidin, stop solution, plate cover and a user protocol.