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Fluorescent Dyes  >  Fluorescent Biopolymers  >>  SMCC Activated R - PE (R - Phycoerythrin)

Product Name SMCC Activated R - PE (R - Phycoerythrin)
Size 1 mg
Catalog # 72110
US$ $133
Description

R-Phycoerythrin (R-PE), a fluorescent protein from phycobiliprotein family, is isolated from red algae. SMCC Activated R-PE is chemically modified with SMCC. SMCC reacts with the primary amine on R-PE and introduces maleimide groups to R-PE. These maleimide groups easily react with thiol groups of target protein without the need for any additional activation, resulting in convenient conjugation of R-PE with proteins. R-PEs primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins with orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. R-PE conjugates are widely used in applications such as flow cytometry, live cell staining, and immunofluorescent staining.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage 4C, Do NOT freeze!
References Huang B, et al. (2002). The experimental research of R-phycoerythrin subunits on cancer treatment: a new photosensitizer in PDT. Cancer Biother Radiopharm 17, 35-42. Galland-Irmouli AV, et al. (2000). One-step purification of R-phycoerythrin from the red macroalga Palmaria palmata using preparative polyacrylamide gel electrophoresis. J Chromatogr B Biomed Sci Appl 739, 117-23.
Spectral Properties Abs/Em = 565/575 nm
     
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