R-Phycoerythrin (R-PE), a fluorescent protein from phycobiliprotein family, is isolated from red algae. SMCC Activated R-PE is chemically modified with SMCC. SMCC reacts with the primary amine on R-PE and introduces maleimide groups to R-PE. These maleimide groups easily react with thiol groups of target protein without the need for any additional activation, resulting in convenient conjugation of R-PE with proteins. R-PE’s primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins with orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. R-PE conjugates are widely used in applications such as flow cytometry, live cell staining, and immunofluorescent staining.
Huang B, et al. (2002). The experimental research of R-phycoerythrin subunits on cancer treatment: a new photosensitizer in PDT. Cancer Biother Radiopharm 17, 35-42. Galland-Irmouli AV, et al. (2000). One-step purification of R-phycoerythrin from the red macroalga Palmaria palmata using preparative polyacrylamide gel electrophoresis. J Chromatogr B Biomed Sci Appl 739, 117-23.