The Tat FRET Peptide has a flexible structure and shows efficient quenching of the dye when TAR RNA is absent. On binding to TAR RNA, a 2.6 fold fluorescence gain is observed as a result of conformational changes in the FRET peptide. TAR is a 59 nt RNA stem-loop structure that forms the 5'-end of all HIV-1 transcripts
Storage
-20°C
References
Göbel, MW. et al. Eur. J. Org. Chem.2008, 1608 (2008).
Molecular Weight
2708.1
Sequence (One-Letter Code)
5-FAM-AAARKKRRQRRRAAAC(TAMRA-Mal)-NH2
Sequence (Three-Letter Code)
5 - FAM - Ala - Ala - Ala - Arg - Lys - Lys - Arg - Arg - Gln - Arg - Arg - Arg - Ala - Ala - Ala - Cys - (TAMRA - Mal) - NH2
