Anti - Keratin - 10 (CT), human Antibody polyclonal
Species Reactivity: Human only
Application: ELISA, WB
Product Description: This polyclonal Anti-Keratin-10 (CT) antibody is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl (0.2 mg/µl) of Phosphate Buffered Saline (pH 7.4) containing 0.05% Sodium Azide.
Immunogen: Rabbit polyclonal Anti-Keratin-10 (CT) antibody was raised against a synthetic peptide derived from the C-terminus (CT) of human Keratin-10.
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.
ELISA for immunizing peptide: 1:5,000-20,000 WB: 1:500-1:2,000 IHC (recommended but not tested): 1:50-100
Figure 1. Western blot of human Keratin-10 in HT-29 cell lysate (Lane A) and SCC-13 cell lysate (Lane B) labeled with the Anti-Keratin-10 (CT), human antibody (Cat# 55073) at a 1:1000 dilution. An immunoreactive band at ~56 kDa was detected in both lysates. (Data kindly provided by Dr. Bishr Omary, University of Michigan).
Figure 2. Western blot of human Keratin-10 in A431 whole cell lysate labeled with the Anti-Keratin-10 (CT), human antibody (Cat# 55073) at 1:200 dilution.