Product Description: Rabbit polyclonal Anti-Keratin-13 (CT) antibody is supplied as an epitope-affinity purified rabbit 50 µg in 250 µl (0.2 mg/ml) of 1X PBS (pH7.4) containing 0.05% sodium azide.Immunogen: Rabbit polyclonal Anti-Keratin-13 (CT) antibody was raised against a synthetic peptide derived from the C-terminus (CT) of mouse keratin-13.
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.
ELISA for immunizing peptide: 1:5,000-20,000 WB: 1:500-1,000 IHC (recommended but not tested): 1:50-100
Figure 1. Western blot of Keratin-13 in mouse esophagus tissue lysate (Lane A) and mouse ear lysate (Lane B) were probed with Anti-Keratin-13 (CT), mouse antibody (Cat# 55077) at 1:1000 dilution. (Data kindly provided by Dr. Bishr Omary at the University of Michigan).