Anti - Keratin - 13 - alpha (CT), human Antibody polyclonal
Species Reactivity: Exclusive to human. Reactivity was validated by ELISA. Specificity was confirmed by Western blot in HT-29 and SCC-13 cell lysates.
Application: ELISA, WB
Product Description: Rabbit polyclonal Anti-Keratin-13α (CT) is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl (0.2 mg/µl) of Phosphate Buffered Saline (pH 7.4) containing 0.05% Sodium Azide.
Immunogen: Rabbit polyclonal Anti-Keratin-13α (CT) was raised against a synthetic peptide derived from the C-terminal (CT) sequence of human keratin 13α.
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.
ELISA for immunizing peptide: 1:5,000-20,000 WB: 1:500-2,000
Figure 1. Western blot of human Keratin-13α in HT-29 cell lysate probed with the Anti-Keratin-13-alpha (CT), human antibody (Cat# 55078) at 1:1,000 dilution. An immunoreactive band at about 51 kDa was detected. (Data kindly provided by Dr. Bishr Omary at the University of Michigan).
Figure 2. Western blot of human Keratin-13α in A431 whole cell lysate labeled with the Anti-Keratin-13-alpha (CT), human antibody (Cat# 55078) at 1:200 dilution. An imunoreactive band at about 51 kDa was detected.