Species Reactivity: Human, rat and mouse. Reactivity of Anti-Caspase 3 (p12) was confirmed by ELISA and dot blot. Specificity was confirmed by Western blot analysis in 3T3 cell lysate.
Application: ELISA, WB
Product Description: This polyclonal Anti-Caspase 3 antibody is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl (0.2 mg/µl) of Phosphate Buffered Saline (pH 7.4) containing 0.05% Sodium Azide.
Immunogen: Rabbit polyclonal Anti-Caspase (p12) antibody was raised against a synthetic peptide derived from the p12 domain of human Caspase 3 preproprotein (GenBank accession #: NP_004337).
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.
ELISA for immunizing peptide: 1:5,000-20,000 WB: 1:500-1:1,000 IHC (recommended but not tested): 1:200
Figure 1. Western blot of Caspase 3 (p12) in HEK293 cell lysate labeled with Anti-Caspase 3 (p12) antibody (Cat# 55040). An immunoreactive band at ~32kDa was detected in the lysate. Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte Fluor 750-labeled secondary antibody (Cat# 61056-05-H750 or 61056-1-H750) was used and the signals detected using an infrared-imaging system (Odyssey, Li-Cor).
Figure 2. Western blot of Caspase 3 (p12) in 3T3 cell lysate labeled with Anti-Caspase 3 (p12) antibody (Cat# 55040). An immunoreactive band at ~32kDa was detected in the lysate (Lane A), this band was blocked by the immunizing peptide (Lane B).