Product Description: This polyclonal Anti-Caspase 3 antibody is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl (0.2 mg/µl) of Phosphate Buffered Saline (pH 7.4) containing 0.05% Sodium Azide.
Immunogen: Rabbit polyclonal Anti-Caspase (p17) antibody was raised against a synthetic peptide corresponding to the sequence of human Capase-3 (…VSKEDHS…). This sequence is also found in mouse and rat.
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.
ELISA for immunizing peptide: 1:5,000-20,000 WB: 1:500-2,000 IHC (recommended but not tested): 1:200
Figure 1. Western blot of Caspase 3 (p17) in 3T3 cell lysate labeled with Anti-Caspase 3 (p17) antibody (Cat# 55041). An immunoreactive band at ~17kDa was detected.