Product Description: Anti-beta-Actin (NT) rabbit polyclonal antibody is supplied as an epitope-affinity purified rabbit IgG 50 µg in 250 µl (0.2 mg/ml) of 1x PBS (pH 7.4) containing 0.05% sodium azide.
Immunogen: Rabbit polyclonal anti-beta-actin (NT) was raised against a 21 amino acid peptide from near the N-terminus (NT) of human beta-actin (Genbank accession NP_001092.1).
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentration for specific application should be determined by the investigator.
ELISA for immunizing peptide: 1: 5,000-20,000 Western blot:1: 500-1,000
Figure 1. IHC of β-actin in HeLa cells stained with rabbit polyclonal Anti-beta-Actin (NT) antibody (Cat# 54591) at 1 µg/ml.
Figure 2. Western blot of β-actin in 3T3 cell lysate probed with rabbit polyclonal Anti-beta-Actin (NT) antibody (Cat# 54591). An immunoreactive band at ~42 kDa was detected (Lane A); the band was blocked by the immunizing peptide (Lane B). Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte Fluor™ 750-labeled secondary antibody (Cat# 61056-05-H750 or 61056-1-H750) was used and the signals detected using an infrared-imaging system (Odyssey, Li-Cor).
Figure 3. Western blot of β-actin in 3T3 cell lysate (Lane A) and mouse heart tissue lysate (Lane B) probed with rabbit polyclonal Anti-beta-Actin (NT) antibody (Cat# 54591). Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte Fluor™ 750-labeled secondary antibody (Cat# 61056-05-H750 or 61056-1-H750) was used and the signals detected using an infrared-imaging system (Odyssey, Li-Cor).
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