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Host: Rabbit Species Reactivity: Human, mouse, rat; others not tested. Application: WB Product Description: Rabbit polyclonal anti-p53 (CT) antibody is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl 1x PBS (pH 7.4) containing 0.05% sodium azide. Immunogen: Rabbit polyclonal anti-p53 (CT) antibody was raised against a synthetic peptide (KKGQSTS), corresponding to human p53 and surrounding the C-terminal (CT) amino acids.Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific applications should be determined by the investigator.
ELISA for immunizing peptide: 1: 5,000-20,000 WB: 1: 500-2,000