Product Description: Rabbit polyclonal Anti-p53 (pSer 15) antibody is supplied as an epitope affinity purified rabbit IgG, 25 µg in125 µl of 1x PBS (pH 7.4) containing 0.05% sodium azide. This antibody was evaluated for specificity with a dot blot assay using synthetic p53 peptides. It only recognized the phosphorylated serine 15 of human p53, not other phosphorylated sites or non-phosphorylated p53.An antibody raised against the non-phosphorylated version of the sequence is available – Rabbit polyclonal Anti-p53 (Ser) antibody, Cat# 28011.
Immunogen: A synthetic peptide corresponding to human p53 surrounding the phosphorylated site of Serine 15 (PPLpSQET) was used as the immunogen.
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.
ELISA for immunizing peptide: 1:5,000-20,000 Western blot:1: 500-2,000 IP (not tested): 3.0-5.0 µg/extract from 107 cells
Figure 1. Western blot of phosphorylated human p53 in hydroxyrea treated Cos-7 cell lysate probed with the Anti-p53 (pSer15) antibody (Cat# 28010). An immunoreactive band was detected at 53 kDa in the lysate.