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Product Description: This rabbit polyclonal anti-CD3 epsilon antibody is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl of 1X PBS (pH 7.4) containing 0.05% sodium azide.Immunogen: Rabbit polyclonalanti-CD3-epsilon antibody was raised against a synthetic peptide corresponding to the C-terminus of human CD3 protein (epsilon chain).Species Reactivity: This epitope affinity purified rabbit polyclonal anti-CD3 epsilon antibody is human, mouse, rat, and chicken reactive. Application: The following concentration ranges are recommended starting points for this product. Optimal working concentrations should be determined by the investigator for specific applications. WB: 0.5-2.0 µg/mlIHC: 1:100 for 10 min at room temperature
Figure1. IHC shows a positive cell membrane staining observed in formalin-fixed human tonsil tissue. Figure 2. Western blot analysis showed a single band at ~22kDa in Jurkat cell lysate when labeled with with the anti-CD3 epsilon antibody (cat# 29588)..