Antibody and streptavidin conjugates of alkaline phosphatase are extensively used as detection reagents in ELISA. The SensoLyte® Luminescent Alkaline Phosphatase ELISA Assay Kit provides a highly sensitive chemiluminescent substrate for quantifying alkaline phosphatase activity in ELISA. The kit contains: • Chemiluminescent alkaline phosphatase substrate • Assay buffer • Wash buffer • Stop solution • An optimized ELISA protocol