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Peptides  >  Exendins  >>  Fluorescein-Trp25-Exendin-4 (FLEX)

Product Name Fluorescein - Trp25 - Exendin - 4 (FLEX)
HGEGTFTSDLSKQMEEEAVRLFIE - (Trp - S - FAM) - LKNGGPSSGAPPPS - NH2
Size 0.5 mg
Catalog # AS-63899
US$ $303
Purity % Peak Area By HPLC ≥ 95%
Description

This peptide is Exendin-4 labeled with fluorescein at the Tryptophan residue. FLEX is equipotent to GLP-1(7-36)-amide and exendin-4 as an inhibitor of [125I] GLP-1 binding to the human GLP-1 receptor stably expressed in CHO cells, and maintains full biological potency and efficacy as measured by the stimulation of cAMP accumulation in these cells. FLEX binding to CHO/hGLP-1R membranes results in an increase in fluorescence anisotropy. The binding is specific and saturable (Kd = 2.0 +/- 0.4 nM), and GLP-1(7-36)-amide and Exendin-4 are equipotent inhibitors of FLEX binding to the human GLP-1 receptor. Thus, FLEX is a potent, biologically active ligand that is useful for the study of the binding and functional characteristics of the human GLP-1 receptor.

Storage -20C
References Chicchi, GG. et al. Peptides 18, 319 (1997), doi: 10.1016/S0196-9781(96)00320-8
Molecular Weight 4606.4
Sequence
(One-Letter Code)
HGEGTFTSDLSKQMEEEAVRLFIE-(Trp-S-FAM)-LKNGGPSSGAPPPS-NH2
Sequence
(Three-Letter Code)
H - His - Gly - Glu - Gly - Thr - Phe - Thr - Ser - Asp - Leu - Ser - Lys - Gln - Met - Glu - Glu - Glu - Ala - Val - Arg - Leu - Phe - Ile - Glu - (Trp - S - FAM) - Leu - Lys - Asn - Gly - Gly - Pro - Ser - Ser - Gly - Ala - Pro - Pro - Pro - Ser - NH2
Product Citations Rajan, S. et al. (2015). Chronic hyperglycemia downregulates GLP-1 receptor signaling in pancreatic β-cells via protein kinase A. Mol Metabolism 4, 265. doi: http://dx.doi.org/10.1016/j.molmet.2015.01.010.
     
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