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Peptides  >  Bacterial Peptides  >>  Bacterial Sortase Substrate III, Abz/DNP

Product Name Bacterial Sortase Substrate III, Abz/DNP
Abz - LPETG - K(Dnp) - NH2
Size 1 mg
Catalog # 63717
US$ $237
Purity % Peak Area By HPLC ≥ 95%
Description

The Staphylococcus aureus transpeptidase Sortase A (SrtA) anchors virulence and colonization-associated surface proteins to the cell wall. SrtA selectively recognizes a C-terminal LPXTG motif. SrtA readily reacts with its native substrate Abz-LPETG-Dap(DNP)-NH2, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this FRET substrate can be monitored at Ex/Em=320 nm/420 nm.

Storage -20C
References Bentley, M. et al. J. Biol. Chem. 282, 6571 (2007).
Molecular Weight 928.0
Sequence
(One-Letter Code)
Abz-LPETG-K(Dnp)-NH2
Sequence
(Three-Letter Code)
Abz - Leu - Pro - Glu - Thr - Gly - Lys(Dnp) - NH2
     
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